Egg extracted from fertile women for using it in assisted reproduction treatment to provide an opportunity to infertile women to conceive. Excess donated eggs can be also used for biological research purposes. But before perusing the egg extraction process, application and screening test require to conduct for the ethical purpose. Egg donor needs to apply for an egg donation program. Based on this application, egg donation service organization takes the interview to the egg donor after an initial screening. After, this initial phase, some tests are conducted to check the general physical condition of the donor. The candidates who pass all the screening tests can only be selected for egg donation process. Following are the different steps follows in egg donation methods:
Matching the criteria
The intended parents first select the egg donor from the pool, who is the best match according to their criteria. Depending upon the availability of both intended parents and donor, IVF coordinator fixed the date to pursue the egg donation process.
Suppression and stimulation of the ovary
The donor may require to suppress her natural cycle for synchronizing the date with the recipient. Synthetic gonadotropin-releasing hormone (Lupron) require to inject daily for suppression of the natural cycle. During ovarian stimulation, the donor needs to parentally administer gonadotropin injections. Only one egg matures through the natural cycle, but gonadotropin injections persuade more numbers of egg maturation for retrieval purpose. Egg donor needs to visit regularly during the ovarian stimulation process to monitor the ovarian response. Ultrasounds and blood tests are conducted to check the condition and test reports help to avoid ovarian hyperstimulation condition.
Preparing endometrial lining of the recipient
Endometrial lining development in the ovary of the recipient is the concurrent step with ovarian stimulation of the donor. Reproductive experts consider 7 mm of endometrial lining in the ovary provides a favorable condition for implantation of egg and support further development of the embryo. Estrogen and progesterone hormones containing medications are prescribed to the recipient for developing a favorable uterine environment with proper thickness of the endometrial lining.
Triggering ovulation and retrieval of egg
After completion of egg development in the donor ovary, a confirmation test is conducted by ultrasound doctor prescribe Human Chorionic Gonadotropin (hCG) injection to the donor for triggering ovulation. The egg retrieval process is conducted after two days of the drug administration in the doctor's clinic. The egg retrieval process is conducted under medical supervision. Sedative medication pushes to the donor before the egg retrieval process. Medical experts insert an ultrasound-guided aspiration needle trans-vaginally to retrieve the eggs. After completion of the process, the donor needs to take rest for a day for recovery purpose.
Fertilization and embryo transfer
The retrieved eggs are going for in-vitro fertilization in the IVF-laboratory. Collected sperm from the partner or sperm donor used for fertilization of the retrieved eggs. After completion of egg fertilization, the created embryo is incubated and graded. In normal cases, the embryo is transferred to the recipient uterus on the third day of egg retrieval, however, in rare cases, it may extend up to 5 days.
Medical checkup of the egg donor and pregnancy test for the recipient
Thorough medical checkup of the donor is necessary for confirmation of complete recovery from ovarian stimulation and egg retrieval procedure. Usually, donor completely recovers after one or two days of egg retrieval procedure conduction. The whole egg donation process is completed within 3 to 5 weeks.
A pregnancy test is also conducted on the recipient after 15 days of the embryo transfer and confirm the successful egg retrieval and implantation process.